Multi-Angle Light Scattering (MALS) measures the amount of light scattered by particles in solution relative to the angle of the incident light. For larger macromolecules and complexes (above 10 nm for 690 nm light) or for unfolded or strongly elongated proteins,聽the angular dependence in a multi-angle light scattering experiment can be used to calculate Rg. Rg is the 鈥渞oot means square radius鈥 or 鈥渞adius of gyration鈥 and reflects the mass distribution of a macromolecule around its center of mass.