Shen Lab Selected Publications
(# equal authorship)
Wan, C., Puscher, H., Ouyang, Y., Wu, J., Tian, Y., Li, S., Yin, Q.,听Shen, J. (2024) An AAGAB-to-CCDC32 handover mechanism controls the assembly of the AP2 adaptor complex. Proc Natl Acad Sci U S A. Aug 20;121(34):e2409341121. PMID: 39145939.
- 颁辞尘尘别苍迟补谤测:听Hollopeter G. (2024) Stepwise assembly of the AP2 endocytic clathrin adaptor complex. Proc Natl Acad Sci U S A. 2024 Sep 17;121(38):e2415313121. PMID: 39250673.听
Gaikwad, H., Siegel, D., Barnes, A., Kanada, M., Wan, C.,听Shen, J., Balyasnikova, I.V., Simberg, D. (2023) Cyanine lipids promote the shedding of extracellular vesicles from cell membranes. Precision Nanomedicine. Vol 6; Issue 3, 1066-1077.听
Wang, S., Chen, X., Crisman, Lauren., Dou, X., Winborn, C.S., Wan, C., Puscher, H., Yin, Q., Kennedy, M.J., and Shen, J. (2023) Regulation of cargo exocytosis by a Reps1-Ralbp1-RalA module. Science Advances.Feb 22;9(8):eade2540. PMID: 36812304.听
Yu, H., and Shen, J. (2023) Faithful SM proteins chaperone SNAREs on path to successful assembly.听 Proc Natl Acad Sci U S A. Jan 17;120(3):e2219769120. PMID: 36623199. (Review)
Tian, Y., #, Datta, I. #, Yang, R. #, Wan, C., Wang, B., Crisman, L., He, H., Brautigam, C.A., Li, S., Shen,听J., and Yin, Q. (2023) Oligomer-to-monomer transition underlies the chaperone function of AAGAB in AP1/AP2 assembly. Proc Natl Acad Sci U S A. Jan 10;120(2):e2205199120. PMID: 36598941. (J. Shen and Y. Qin are both corresponding authors)
Wang, S., Wan, C., Squiers, G.T., Shen, J. (2022) Endocytosis Assays Using Cleavable Fluorescent Dyes. Methods Mol Biol. 2022;2473:181-194. PMID: 35819767.
Wan, C., Stowell, M.H.B., and Shen, J. (2022) Studies of extracellular vesicle-mediated intercellular cargo transfer in the central nervous system 鈥 progress and gaps. Communication Biology.听Nov 11;5(1):1223. PMID:听36369335. (Review)
Wan C.#, Crisman L.#, Wang B, Tian Y, Wang S, Yang R, Datta I, Nomura T, Li S, Yu H, Yin Q, Shen J. (2021) AAGAB is an assembly chaperone regulating AP1 and AP2 clathrin adaptors. J Cell Sci. Sep 8:jcs.258587. doi: 10.1242/jcs.258587. PMID: 34494650. 听
Liu, Y.#, Wan, C.#, Rathore, S.S., Stowell, M.H.B., Yu, H., and Shen, J. (2021) SNARE zippering is suppressed by a conformational constraint that is removed by v-SNARE splitting. Cell Reports.Jan 12;34(2):108611.
Zhang, X., Xu, Q., Zi, Z., Liu, Z., Wan, C., Crisman, L., Shen, J., Liu, X. (2020) Programmable Extracellular Vesicles for Macromolecule Delivery and Genome Modifications. Dev Cell. 2020 Dec 1:S1534-5807(20)30883-2.
Dombach, J.L., Quintana, JLJ, Nagy, T.A., Wan, C., Crooks, A.L., Yu, H., Su, C.C., Yu, E.W., Shen, J., Detweiler, C.S. (2020) A small molecule that mitigates bacterial infection disrupts Gram-negative cell membranes and is inhibited by cholesterol and neutral lipids. 听PLoS Pathog. 2020 Dec. 8;16(12):e1009119.
Menasche, B.L. #, Davis, E.M. #, Wang, S.#, Ouyang, Y., Li, S., Yu, H., Shen, J. (2020) PBRM1 and the glycosylphosphatidylinositol biosynthetic pathway promote tumor killing mediated by MHC-unrestricted cytotoxic lymphocytes. Science Advances. Nov 27;6(48):eabc3243.
Wang, S., Liu, Y., Crisman, L., Wan, C., Miller, J., Yu, H., Shen, J. (2020) Genetic evidence for an inhibitory role of tomosyn in insulin-stimulated GLUT4 exocytosis. Traffic. Oct;21(10):636-646. doi: 10.1111/tra.12760. PMID: 32851733.
Yang, C., Chen, F., Ren, P., Lofchy, L., Wan, C., Shen, J., Wang, G., Gaikwad, H., Ponder, J., Jordan C.T., Scheinman, R., Simberg, D. (2020) Delivery of a model lipophilic membrane cargo to bone marrow via cell-derived microparticles. J Control Release. Oct 10;326:324-334.
Zhang, Q., Xu, Y., Lee, J., Jarnik, M., Wu, X., Bonifacino, J.S., Shen, J., Ye, Y. (2020) A myosin-7B-dependent endocytosis pathway mediates cellular entry of 伪-synuclein fibrils and polycation-bearing cargos. Proc Natl Acad Sci U S A. May 19;117(20):10865-10875. PMID: 32366666.
Rathore, S.S.#, Liu, Y.#, Yu, H., Wan, C., Lee, M., Yin, Q., Stowell, M.H., Shen, J. (2019) Intracellular vesicle fusion requires a membrane-destabilizing peptide located at the juxtamembrane region of the v-SNARE. Cell Reports. (Article). Dec 24;29(13):4583-4592. PMID: 31875562.
Wang, S.#, Crisman, L. #, Miller, J., Datta, I., Gulbranson, D.R., Tian, Y., Yin, Q., Yu, H., and Shen, J., (2019) Inducible Exoc7/Exo70 knockout reveals a critical role of exocyst in insulin-regulated GLUT4 exocytosis. J. Biol. Chem.Nov. 18. PMID: 31740584.
Gulbranson, D.R.#, Crisman, L.#, Lee, M., Ouyang, Y., Menasche, B.L., Demmitt, B.A., Wan, C., Nomura, T., Ye, Y., Yu, H., and Shen, J.听 (2019) AAGAB controls AP2 adaptor assembly in clathrin鈥恗ediated endocytosis. Dev Cell. 50, 436鈥446. PMID: 31353312.
Yu, H., Crisman, L. Stowell, M.H., and Shen, J. (2019) Functional reconstitution of intracellular vesicle fusion using purified SNAREs and Sec1/Munc18 (SM) proteins.听Methods in Molecular Biology. (Review). 1860:237-249. PMID: 30317509.
Shen, C.#, Liu, Y.#, Yu, H., Gulbranson, D.R., Kogut, I., Bilousova, G., Zhang, C., Stowell, M.H., and Shen, J.(2018) The N-peptide binding mode is critical to Munc18-1 function in synaptic exocytosis. J Biol Chem. Oct 1. pii: jbc.RA118.005254. PMID: 30275014.
Yu, H,, Shen, C., Liu, Y., Menasche, B.L., Ouyang, Y., Stowell, M.H., Shen, J. (2018) SNARE zippering requires activation by SNARE-like peptides in Sec1/Munc18 proteins. Proc Natl Acad Sci U S A. Sep 4;115(36):E8421-E8429. PMID: 30127032.
Menasche, B.L., Crisman, L., Gulbranson, D.R., Davis, E.M., Yu, H., and Shen, J., (2018) Fluorescence activated cell sorting (FACS) in genome-wide genetic screening of membrane trafficking. 听Current Protocols in Cell Biology. (Review). Sep 28:e68. doi: 10.1002/cpcb.68. PMID: 30265447.
Guan, X., Chaffey, P.K. Wei, X. Gulbranson, D.R. Ruan, Y., Wang, X., Li, Y., Ouyang, Y., Chen, L., Zeng, C., Koelsch, T.N., Tran, A.H., Liang, W.*, Shen, J.* and Tan, Z *. (2018)听Chemically Precise Glycoengineering Improves Human Insulin.听ACS Chemical Biology.听 2018 Jan 19;13(1):73-81. PMID: 29090903 (*corresponding authors)
Gulbranson, D.R., Davis, E.M.,听 Demmitt, B.A., Ouyang, Y., Ye, Y., Yu, H., Shen, J. (2017) RABIF/MSS4 is a Rab-stabilizing holdase chaperone required for GLUT4 exocytosis.听Proc Natl Acad Sci U S A.听听听Sep 26;114(39):E8224-E8233. PMID: 28894007.听(Plus article, Highlighted by Faculty of 1000)
Yu, H.,听., Gulbranson, D.R., Paine, A., Rathore, S.S., and Shen, J. (2016)听Extended synaptotagmins are Ca2+-dependent lipid transfer proteins at membrane contact sites.听Proc Natl Acad Sci U S A.听 April 4. PMID: 27044075.
Shen, C.#, Rathore, S.S.#, Yu, H.#, Gulbranson, D.R., Hua, R., Zhang, C., Schoppa, N.E., and Shen, J., (2015) The trans-SNARE-regulating function of Munc18-1 is essential to synaptic exocytosis.听Nature Communications.听6:8852. PMID:26572858
Yu H.#, Rathore, S.S.#, Shen, C., Liu, Y., Ouyang, Y., Stowell, M.H., and Shen, J. (2015) Reconstituting intracellular vesicle fusion reactions: the essential role of macromolecular crowding.听J Am Chem Soc.听(Article) 137(40):12873-83.
Davis EM, Kim J, Menasche BL, Sheppard J, Liu X, Tan AC, Shen J. (2015) Comparative Haploid Genetic Screens Reveal Divergent Pathways in the Biogenesis and Trafficking of Glycophosphatidylinositol-Anchored Proteins. Cell Reports.(Article) Jun 10. pii: S2211-1247 (15) 00552-5.
Zhang C., Lee, S., Peng, Y., Bunker, E., Shen, C., Giaime, E., Shen, JI., Shen, J.,听 Zhou, Z., and Liu, X. (2015) A Chemical Genetic Approach to Probe the Function of PINK1 in Regulating Mitochondrial Dynamics.听Cell Research.听Mar;25(3):394-7.
Yu, H, Rathore, S.S., Gulbranson, D.R., Shen, J., (2014) The N- and C-terminal domains of tomosyn play distinct roles in SNARE binding and fusion regulation.听 J Biol Chem.听听pii: S0960-9822(14)00840-9. PMID: 25063806.
Shen, J. (2013) New tools for old questions - Voices on Directing Traffic into the Future.听Developmental Cell.听27 (5) 483.
Yu, H., Rathore, S.S., Lopez, J.A., Davis, E.M., James, D.E., Martin, J.L., Shen, J. (2013) Comparative studies of Munc18c and Munc18-1 reveal conserved and divergent mechanisms of Sec1/Munc18 proteins.听Proc Natl Acad Sci U S A.听 110 (35) E3271-E3280. PMID: 23918365. (*PNAS Plus Article)
- Commentary: Shin, Y.K., (2013) Two gigs of Munc18 in membrane fusion.听Proc Natl Acad Sci U S A.听110 (35) 14116-14117. PMID: 23946422.
Yu, H., Rathore S.S., Shen, J. (2013) Synip Arrests Soluble N-Ethylmaleimide-sensitive Factor Attachment Protein Receptor (SNARE)-dependent Membrane Fusion as a Selective Target Membrane SNARE-binding Inhibitor.听J Biol Chem.Jun 28;288(26):18885-93. PMID: 23665562.
Yu, H., Rathore, S.S., Davis, E. M., Ouyang, Y., and Shen, J. (2013) Doc2b promotes GLUT4 exocytosis by activating the SNARE-mediated fusion reaction in a calcium- and membrane bending-dependent manner.听Mol Biol Cell.听Apr;24(8):1176-84. PMID: 23427263.
Rathore, S.S., Ghosh, N., Ouyang, Y., Shen, J. (2011) Topological restriction of intracellular membrane fusion machinery.Mol Biol Cell,听(14) 2612-9. PMID: 21633111
Rathore, S. S.#, Bend, E.G.#, Yu, H., Hammarlund, M., Jorgensen, E.M., Shen, J. (2010) Syntaxin N-terminal peptide motif is an initiation factor for the assembly of the SNARE鈥揝ec1/Munc18 membrane fusion complex.听Proc Natl Acad Sci U S A.107 (52), 22399鈥22406. PMID: 21139055.听 (Cover story and the only feature article in the issue)
- Commentary: Bacaja, T., Panga, Z. P., and S眉dhof, T.C., (2010) Testing the SNARE/SM Protein Model of Membrane Fusion. Proc Natl Acad Sci U S A. 107听 (52) 22365-22366. PMID: 21169506.
Shen, J*, Rathore, S.S., Khandan, L., Rothman, J.E. (2010). SNARE bundle and syntaxin N-peptide constitute a minimal complement for Munc18-1 activation of membrane fusion.听J Cell Biol.听190(1):55-63. PMID: 20603329.听(* corresponding author)听
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